FSBI "ARRIAH" has developed the first kit in Russia for diagnosing foot-and-mouth disease serotype SAT-2 using enzyme-linked immunosorbent assay

The institution already has a foot-and-mouth disease vaccine against serotype SAT-2 in its reserve, and today employees of the foot-and-mouth disease prevention laboratory announced the first development in RUSSIA - the creation of a diagnostic test system for determining antibodies to the structural proteins of the foot-and-mouth disease virus serotype SAT-2 based on a liquid-phase blocking version of ELISA.

The development included the production of reaction components: foot-and-mouth disease virus antigen SAT-2, sensitizing (capturing) and detector antibodies to structural proteins of foot-and-mouth disease virus serotype SAT-2, immunoperoxidase conjugate, control blood serum samples; determination of working dilutions of specific reaction components with their subsequent lyophilization. The kit was tested and evaluated for the determination of antibodies to the structural proteins of foot and mouth disease virus serotype SAT-2.

This test system allows you to detect specific antibodies to the structural proteins of the foot-and-mouth disease virus serotype SAT-2 in the blood serum of vaccinated or recovered animals. Based on a statistical analysis of the ELISA results, the high diagnostic characteristics of the new kit were determined (sensitivity - no less than 97.01%, specificity - no less than 96.36%). Thanks to the proposed diagnosticum, it is possible to conduct screening studies of blood serum, assess the strength of immunity, and monitor the immunogenicity of foot-and-mouth disease vaccines. The developed kit also makes it possible to determine the antibody titer by the method of serial dilutions with the determination of the titration end point or serum titer with a high degree of reliability of the analysis.

In addition, on behalf of the OIE Reference Laboratory and the FAO Reference Center for Foot and Mouth Disease (FSBI "ARRIAH"), information letters were sent about the high risks of introducing the foot and mouth disease virus serotype SAT-2 into the countries of Transcaucasia (Armenia, Azerbaijan, Georgia) and Central Asia ( Kazakhstan , Kyrgyzstan, Uzbekistan , Turkmenistan and Tajikistan), as well as the readiness to provide scientific and methodological assistance in the sampling of biomaterial, training of veterinary specialists, emergency laboratory testing of blood sera and biomaterial of susceptible (sick) animals.