PIONEER MEIZHENG BIO-TECH (5 in1) JC0586 - Antibiotic tests 5 in 1 / Rapid tests for determining the residual amount of β-lactams, tetracyclines and cephalexin in milk, whey

26.07.2020
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Rapid tests to determine residues

β-lactams, tetracyclines, chloramphenicol, streptomycins, cephalexin

in Milk , whey.

 
  • Detection limits fully comply with the technical regulations of the Customs Union TR CU 033/2013, SanPiN No. 52 dated June 21, 2013, and the legislation of the European Union, at the levels regulated by the Decision of the Eurasian Economic Commission No. 28 dated February 13, 2018.
  • Validated by the state independent accredited laboratory of the Republic of Belarus, part of the Gosstandart structure

 

  • PRODUCTS OF ANIMAL ORIGIN. Method for detecting residual amounts of beta-lactams, tetracyclines, chloramphenicol, streptomycins, cephalexin in milk, whey using PIONEER MEIZHENG BIO-TECH test kits (5 in 1) JC0586

 

General information:

 

The kit is used for the determination of β-lactams, tetracyclines, chloramphenicol, streptomycins, cephalexin (penicillin G, cloxacillin , amoxicillin , oxacillin , nafcillin , ampicillin , dicloxacillin , benzathine penicillin (benzicillin),   hetacillin , piperacillin , proca in-penicillin , ticarcillin , cefkin , cephalonium , ceftiofur, cefapirin, cefoperazone , cefacetril, cefazolin, cefadroxil, cefoxazole, cefotaxime , cefuroxime , tetracycline , chlortretracycline , doxycycline , oxytetracycline, streptomycin, dihydrostreptomycin , chloramphenicol , cephalexin ) in raw, pasteurized, sterilized, reconstituted milk powder, whey, whey dry reconstituted milk.

Storage conditions:

Test kits should be stored at 2-8 °C. Do not freeze!

Shelf life: 12 months. The batch number and expiration date are indicated on the packaging.

Operating principle:

  • The set of reagents uses an immunochromatographic analysis method using colloidal gold particles. The sample is added to the antibody well; if antibiotics are present in the sample , they will bind to the antibodies, thus preventing the subsequent binding of antibodies to antigens coated on the nitrocellulose membrane of the test strip. The result of the reaction is the coloring of the strips, which is taken into account subsequently.

 

Test kit detection limit:

 

Table 1

 

β-lactams

 

Penicillins

Detection limit

Cephalosporins

Detection limit

 

ppb (µg/l)

ppb (µg/l)

 

 

 

 

Penicillin G

1-2

Tsefkin

5-7

 

Cloxacillin

2-5

Cephalonium

3-5

 

Amoxicillin

2-3

Ceftiofur

60-80

 

Oxacillin

4-6

Cefapirin

4-8

 

Nafcillin

7-9

Cefoperazone

3-5

 

Ampicillin

2-3

Cefacetril

10-20

 

Dicloxacillin

2-5

Cefazolin

15-25

 

Benzathine penicillin (Benzicillin)

2-4

Cefadroxil

10-15

 

Hetacillin

5-8

Cefoxazole

70-90

 

Piperacillin

5-6

Cefotaxime

25-30

 

Procaine-penicillin

2-4

Cefuroxime

25-30

 

Ticarcillin

3-6

Cephalexin

10-15

 

Tetracyclines

Detection limit

Tetracyclines

Detection limit

 

ppb (µg/l)

ppb (µg/l)

 

 

 

 

Tetracycline

6-8

Doxycycline

3-4

 

Chlortetracycline

5-7

Oxytetracycline

7-9

 

Other antibiotics

ppb (µg/l)

Other antibiotics

ppb (µg/l)

 

 

 

 

 

 

Streptomycin

50

Dihydrostreptomycin

50

 

Chloramphenicol

0.2-0.3

 

 

 

 

Set contents: The set includes everything needed for 96 definitions:

  • 12 tubes, each of which contains 1 strip with 8 wells with a reagent containing antibodies, and 8 test strips;
  • 100 disposable tips (pipette dispenser 200 µl);
  • Instructions;
  • 8 wells with a reagent for control - Positive standard (the concentration of the antibiotic is indicated on the packaging of the positive control and in the quality certificate that comes with each batch of test kits);

- a bottle with a reagent for negative control (does not contain antibiotics );

  • 1 well plate;
  • cards with QR codes for calibrating the BMZ6000 reader (see the instructions for the reader).

Sample preparation of milk and whey samples:

Powdered milk , powdered whey: in a suitable flask, dissolve the sample with warm distilled water (according to the current regulatory documentation), mix thoroughly.

Reconstituted milk powder , reconstituted whey powder: samples must be liquid and homogeneous. The samples should be free of clots and product separation phases. The sample temperature must be at least 4 °C, not frozen or heated. Mix the sample thoroughly before testing.

Preparing the Mini-T type incubator:

The analysis can be carried out either with or without an incubator.

When using an incubator, do the following: Place the incubator on a workbench with a flat surface. Connect the power supply to the incubator, and then plug IT into a 220V outlet. Set the switch to the operating position. Next, set the operating temperature to 40 ° C and wait until the device warms up. Once the incubator has reached operating temperature, you can place the sample well in the appropriate hole.

 

Carrying out self-monitoring of the test kit:

 

Before starting to work with samples, you should perform a self-diagnosis of the test kit. To do this, use the negative and positive control samples included in the kit. Further work with a kit with confirmed characteristics and detection limits of antibiotics is permitted if a completely negative result is obtained from the analysis of the negative control sample, and a completely positive result from the analysis of the positive control sample.

  • Preparation of negative control sample:

Add 2 ml of distilled or deionized water to the vial containing the negative control sample and mix thoroughly. The prepared negative standard should be stored at a temperature of 2-8 °C for no more than 24 hours. A frozen negative sample at or below -16°C should be stored in the refrigerator for no more than 30 days. Before use, the negative control should be brought to room temperature and mixed thoroughly. Next, follow the procedure for analyzing milk and whey (read below).

  • Preparation of a positive control sample:

Add 200 µl of the reconstituted negative control sample to the reagent well containing the positive control sample. Using a pipette, mix them thoroughly. After mixing, the sample mixture and the reagent from the well should have a homogeneous structure. Place the well containing the pink antibody reagent into a preheated 40°C incubator or well plate. Pipette 200 µl of the resulting positive control sample and transfer it to the well containing the pink reagent, using the pipette to mix 10 times until the pink color is uniform. Incubate the mixture for 3 minutes. Then place the test strip from the kit into the sample well and incubate for another 7 minutes.

After incubation, remove the test strip from the sample well and interpret the result within 3 minutes.

If the test kit is stored correctly, internal quality control using positive and negative standards may only be performed once before handling the tests, when the test kit box is first opened.

Procedure for analyzing milk and whey:

Read the instruction manual before analysis. Determine how many tests you need, remove the required number of tests from the refrigerator. Kit reagents should be at room temperature. It is advisable to use reagents and test strips from open tubes within 24 hours of removal from the refrigerator to avoid distortion of the results. All kit reagents that are not in use must be stored in the refrigerator. Avoid exposure of reagents to direct sunlight and excess moisture. The reagent in the well is specially dried. Don't think it's spoiled.

  • When using an incubator , place the well with the sample in an incubator preheated to 40 °, use a pipette to take a 200 μl sample of milk or whey and transfer it to the well with the reagent from the kit. Thoroughly mix the sample with the reagent by adding ten times and draining the liquid using a pipette in the well until it turns uniformly pink and incubate the mixture for 3 minutes.

Then place the test strip from the kit into the well with the reagent and sample and incubate in the incubator for another 7 minutes at 40 ° C.

  • In the absence of an incubator , the analysis can be carried out indoors at a temperature of 16-25 ° C. Place the well with the sample in the well plate, use a pipette to take a 200 μl sample of milk or whey and transfer it to the well with the reagent from the kit. Thoroughly mix the sample with the reagent by adding ten times and draining the liquid using a pipette in the well until uniform pink coloring, incubate the mixture for 3 minutes.

Then place the test strip from the kit into the well with the reagent and sample and incubate for another 7 minutes. If the test strip is staining slowly, it is recommended to increase the incubation time by 1 - 2 minutes.

 

After incubation, remove the test strip from the reagent and sample well and interpret the result visually within 3 minutes, or read using the BMZ 6000 reader (if equipped). To save your result, remove the filter from the bottom end
of the test strip.

Visual interpretation of the result:

Negative result (-): the control line (C) is colored red; the test line corresponding to its antibiotic (T1 ... T5) is colored brighter red than the control line (C) or has the same staining intensity as the control line (C). This means that the sample contains no antibiotics at all, or contains less than the detection limit of the test kit (see Table 1).

Positive result (+): the control line (C) is colored red; the test line corresponding to its antibiotic (T1 ... T5) is absent or has a weaker staining intensity compared to the control line (C). This means that the sample contains a band-matched antibiotic greater than the detection limit of the test kit (see Table 1).

Invalid result: the control line (C) did not appear at all. This means that the test was performed incorrectly or the test strip has deteriorated. The test must be repeated.

Note:

 

  • The test kit is a qualitative method for detecting residual amounts of antibiotics in milk and whey.
  • Do not use damaged or expired kit components.
  • After removing the kit from the refrigerator, it is advisable to use the opened components within 24 hours.
  • Hold the test strip only by the upper end. Do not touch the filter on the working surface of the test strip! Do not touch the membrane in the middle of the test strip!
  • Close the tube tightly after removing the required number of test strips and wells.
  • Place the test strips into the wells with the working part with the filter.
  • Each time you collect a new sample, use a new disposable pipette tip to avoid accidental contamination.
  • The test strip is used only once.
  • Milk and whey samples, the mixture of sample and dye in the well must be homogeneous. Examination of milk and whey samples with foreign particles, clots and separation phases may lead to distorted results.
  • If necessary, it is possible to store the test strip with the result for no more than 12 months.
  • One test strip and one well with reagent are used per sample; the remaining test strips and wells are stored in a closed tube at a temperature of 2-8 ° C.

 

 

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