ARRIAH has developed a method for the rapid diagnosis of vesicular stomatitis in animals

Scientists recalled that vesicular stomatitis is a disease of horses, cattle and pigs caused by vesiculoviruses of the Vesiculovirus genus of the Rhabdoviridae family.

“From a clinical point of view, vesicular stomatitis cannot be reliably differentiated from other vesicular diseases in relevant susceptible species, such as foot and mouth disease, porcine vesicular exanthema and porcine vesicular disease. In this regard, early laboratory diagnosis of each case of suspected vesicular stomatitis is relevant,” explained ARRIAH experts.

The World Organization for Animal HEALTH (OIE) also notes that the disease is clinically indistinguishable in animals from foot-and-mouth disease, porcine vesicular exanthema or porcine vesicular disease. Sheep, goats and many other species of wild animals can become infected. Humans are also susceptible to this disease.

The ARRIAH reported that there are no commercial test systems for detecting the vesicular stomatitis virus by PCR on the domestic market.

“The proposed method for detecting and typing the vesicular stomatitis virus is based on a polymerase chain reaction with real-time detection of results (RT-PCR), which is characterized by high sensitivity, specificity and speed of obtaining results,” said experts from the institute.

The method is based on the amplification of highly conserved regions of the genome of the vesicular stomatitis virus of the New Jersey (NJ) and Indiana (IND) serotypes and makes it possible to detect the virus in biomaterial and simultaneously determine its serotype.