All porcine serum samples were negative for antibodies to foot-and-mouth disease virus.

The ELISA method used in the research is an important tool for the serological diagnosis of foot-and-mouth disease and the monitoring of cross-border movements of animals and animal products. International regulations on trade in livestock require testing cattle and pigs for antibodies to non-structural proteins of the foot-and-mouth disease virus. These tests enable the detection of infection regardless of the virus serotype, which is particularly critical for foot-and-mouth disease-free countries that do not vaccinate.