The Federal State Budgetary Institution "VGNKI" has developed a method for determining the mass fraction of quinolones in livestock products

The goal of this work was to optimize chromatographic conditions and mass spectrometric detection parameters, as well as methods for preparing samples for analysis.

Today, this technique makes it possible to determine the desired compounds in the concentration range from 1 to 1000 μg/kg for quinolones and from 1 to 2000 μg/kg for difloxacin and flumequin in by-products. In addition, the list of analyzed substances was expanded to emergent (relatively recently appeared) analytes, such as nadifloxacin, gatifloxacin, cinoxacin, sparfloxacin , orbifloxacin, fleroxacin, gemifloxacin, pazufloxacin, enoxacin , grepafloxacin, which in the future will make it possible to quickly monitor the presence of drugs in livestock products based on connection data.

Quinolones, especially fluorinated ones, are one of the most important broad-spectrum antibacterial drugs in veterinary medicine. Most often, quinolones are used to treat diseases of the urinary, respiratory and digestive systems of farm animals (cattle, small livestock, poultry and others) caused by pathogenic microbes.

Inappropriate use of antibiotics for the prevention and treatment of diseases of farm animals, as a rule, leads to their contamination of livestock products, which, in turn, can have a negative impact on human HEALTH . Among such influences, one can highlight skin allergic phenomena, imbalance of intestinal microbiota, arthrotoxic effects, and increased resistance of pathogenic microorganisms to the drugs used.

It is precisely because of the risk of resistance to antimicrobial drugs, as well as a large number of side effects from their use in RUSSIA and other countries, that regulatory restrictions on the content of various fluoroquinolones and quinolones have been introduced on the territory of the Russian Federation, and maximum permissible levels (MAL) of residues have been established. of these antibiotics in food.

As a result of the research, methodological guidelines were developed and metrological certification of the methodology was carried out. In 2024, the methodology will be included in the scope of accreditation and introduced into the activities of the Testing Center of the Federal State Budgetary Institution "VGNKI".