FSBI "ARRIAH" has developed methodological recommendations for the identification of bacteria of the genus Clostridium

The authors of the guidelines focused on optimizing sample preparation, which allows for efficient processing of various groups of products and feeds. The systematic approaches proposed in the document simplify the process of identifying clostridia, making it more accessible to a wide range of specialists.

In addition to classical methods, the recommendations describe innovative techniques, such as the use of the API 20 A test system and the MALDI-TOF Autof ms 1000 mass spectrometer. These methods significantly speed up the process of identifying Clostridium spp.

Of particular note is the creation of a custom database for the MALDI-TOF Autof ms 1000 mass spectrometer, which includes the spectra of Clostridium botulinum. This achievement fills a gap in commercially available databases and expands the ability to identify one of the world's most dangerous pathogens.

The methodological recommendations are intended for use in organizations and institutions carrying out microbiological studies of food and feed.