General information:
The kit is used to determine bacitracin, ansamycins (rifampicin, rifaximin, rifamycin, rifabutin, rifapentine), clindamycin, pyrlimycin, spiramycin, florfenicol, thiamphenicol in raw, pasteurized, sterilized, reconstituted Milk powder, milk whey, reconstituted dry milk whey.
Storage conditions:
Test kits should be stored at 2-8°C. Do not freeze! Shelf life: 12 months. Lot number and expiration date are indicated on the package.
Operating principle:
Test kit detection limit: |
| Table 1 | |
Antibiotic | Limit of detection ppb (µg/kg) | ||
bacitracin | 40-60 | ||
clindamycin pyrlimycin | 1-2 1-2 | ||
spiramycin | 30-40 | ||
florfenicol | 1-2 3-6 | ||
Ansamycins | Limit of detection ppb (µg/kg) | ||
rifampicin | 4-8 | ||
rifaximin | 40-60 | ||
rifamycin | 30-40 | ||
rifabutin | 30-50 | ||
rifapentine | 10-20 |
Contents of the kit: The kit includes everything you need for 96 definitions:
- negative control reagent bottle (does not contain antibiotics);
Sample preparation of milk and whey samples:
Powdered milk, powdered milk whey: Dissolve the sample in a suitable flask with warm distilled water (according to the current regulatory documentation), mix thoroughly.
Milk , reconstituted milk powder, reconstituted whey powder: Samples must be liquid and homogeneous. Samples should be free of clots and product separation phases. The temperature of the sample must be at least 4°C, neither frozen nor heated. Mix the sample thoroughly before testing.
Preparing the Mini-T Incubator:
The analysis can be carried out with or without an incubator.
When using an incubator, do the following: Place the incubator on a work bench with a flat surface. Connect the power supply to the incubator, and then plug IT into a 220V socket. Set the switch to working position. Next, set the operating temperature to 40 ° C and wait until the device heats up. Once the incubator has reached operating temperature, the sample well can be placed in the appropriate hole.
Carrying out self-testing of the test kit:
Before you start working with samples, you should perform a self-test on the test kit. To do this, use the negative and positive controls included in the kit. Further work is permitted on a kit with validated antibiotic performance and detection limits if a negative control is completely negative and a positive control is completely positive.
Add 2 ml of distilled or deionized water to the negative control vial and mix well. Store the prepared negative standard at a temperature of 2-8 °C for no more than 24 hours. A frozen negative sample at or below -16°C should be stored in a refrigerator for no more than 30 days. Before use, the negative control should be brought to room temperature and mixed thoroughly. Then follow the milk and whey analysis procedure (read below).
Add 200 µl of the reconstituted negative control to the reagent well containing the positive control. Mix thoroughly with a pipette. After mixing, the mixture of sample and reagent from the well should have a homogeneous structure. Place the well with the pink antibody reagent in a preheated 40°C incubator or well plate. Pipette 200 µl of the obtained positive control sample and transfer to the well with the pink reagent, using a pipette mix them 10 times until a uniform pink color is obtained. Incubate the mixture for 2 minutes. Then place the test strip from the kit into the sample well and incubate for another 5 minutes.
After incubation, remove the test strip from the sample well and interpret the result within 3 minutes.
If the test kit is properly stored, it is allowed to perform internal quality control using positive and negative standards only once before starting work with tests when first opening the box of the test kit.
Milk and whey analysis procedure:
Read the instruction manual before analysis. Determine how many tests you need, remove the required number of tests from the refrigerator. Kit reagents should be at room temperature. It is advisable to use reagents and test strips from open tubes within 24 hours from the moment they are removed from the refrigerator, in order to avoid distorting the results. Any unused kit reagents must be refrigerated. Avoid direct sunlight and excessive moisture on reagents. The reagent in the well is specially dried. Don't think it's corrupted.
Then place the test strip from the kit into the well with the reagent and sample and incubate in the incubator for another 5 minutes at 40°C.
Then place the test strip from the kit into the well with the reagent and sample and incubate for another 5 minutes.
After incubation, remove the test strip from the reagent and sample well and visually interpret the result within 3 minutes, or read with the BMZ 6000 reader (if available). To save the result, remove the filter from the bottom end of the
test strip.
Visual interpretation of the result:
Invalid result: the control line (C) did not appear at all. This means that the analysis was not carried out correctly, or the test strip has deteriorated. The test must be repeated.
Note:
Manufacturer : Beijing Meizheng Bio-Tech Co., No.-2 Building, No.-8 Courtyard, Fenggusilu Road, Yanqing District, (Zhongguancun Yanqing Science Park), Beijing; Shandong Meizheng Bio-Tech Co., Ltd. no. 69, Zhaoyang North Road, Rizhao High-Tech Industrial Development Zone, Shandong Province; China