PIONEER MEIZHENG BIO-TECH (3 in 1) JC0585 / Express Tests for the determination of residual quantities of β-lactam, tetracyclines and cephalexin in milk, whey

Novelty 2020
 
General information:
 
The kit is used for the determination of β-lactams, tetracyclines and cephalexin (penicillin G, cloxacillin, amoxicillin, oxacillin, nafcillin, ampicillin, dicloxacillin, benzatine penicillin (benzicillin), getacillin, piperacillin, procain-penicillin, tikarcillin, cefalonium, ceftiofur, cephapirin, cephoperazone, cefacetrile, cefazolin, cefadroxyl, cefoxazole, cefotaxime, cefuroxime, tetracycline, chlorotretracycline, doxycycline, oxytetracycline, cephalexin) in raw, pasteurized, sterilized, reconstituted milk powder, milk whey, milk powder reconstituted.
 
Terms and conditions of storage:
 
Test kits should be stored at a temperature of 2-8 ° C. Do not freeze! Shelf life: 12 months. Batch number and expiry date are shown on the packaging.
 
Principle of operation:
 
  • The reagent kit uses immunochromatographic method using colloidal gold particles. The sample is added to the antibody well, if antibiotics are present in the sample, they will bind to the antibodies, thus preventing subsequent binding of the antibodies to the antigens applied to the nitrocellulose membrane of the test strip. The result of the reaction is a staining of the test strips, which is taken into account subsequently.
 
 

The detection limit of the test set:

 

Table 1

β-lactams

Penicillins

Ppb detection limit (µg/l)

Cephalosporins

Ppb detection limit (µg/l)

Penicillins G

1-2

Cefkin

5-7

Kloxacillin

2-5

Cephalonium

3-5

Amoxicillin

2-3

Ceftiofur

60-80

Oxacillin

4-6

Cefapirin

4-8

Nafcillin

7-9

Cefoperazone

3-5

Ampicillin

2-3

Cefacetrile

10-20

Dicloxacillin

2-5

Cefazolin

15-25

Benzatin penicillin (Benzicillin)

2-4

Cefadroxyl

10-15

Getacillin

5-8

Cefoxazole

70-90

Piperacillin

5-6

Cefotaxime

25-30

Прокаин-пенициллин

2-4

Procain-penicillin

25-30

Ticarcillin

3-6

Cefalexin

10-15

Tetracyclines

Ppb detection limit (µg/l)

Tetracyclines

Ppb detection limit (µg/l)

Tetracycline

6-8

Doxycycline

3-4

Chlorotetracycline

5-7

Oxitetracycline

7-9

 

Set Composition: The set includes everything you need for 96 definitions:
 
 
  • 12 tubes, each containing 1 strip with 8 wells of antibody-containing reagent and 8 test strips;
  • 100 disposable tips (pipette dispenser for 200 µl);
  • Instruction;
  • 8 reagent wells - Positive standard (antibiotic concentration is indicated on the positive control package and on the quality certificate for each batch of test kits);
  • - Reagent bottle for negative control (contains no antibiotics);
  • 1 well tablet;
  • QR-code cards for calibration of the BMZ6000 reader (see instruction manual for the reader).
 
 
Sample preparation of milk and whey:
 
 
Milk powder, whey powder: In a suitable flask, dissolve the sample with warm distilled water (according to current regulations), mix thoroughly.
 
 
 
Milk, reconstituted milk powder, reconstituted whey powder: Samples should be liquid and homogeneous. Samples should be free of clots and product separation phases. The temperature of the sample should be at least 4 oC, not frozen and not heated. Mix the sample thoroughly before testing.
 
 
Preparation of a Mini-T incubator:
 
 
The analysis can be done with or without an incubator.
 
When using an incubator, do the following: place the incubator on a flat surface worktable. Connect the power supply to the incubator and then plug it into a 220V socket. Set the switch to the operating position. Then set the operating temperature to 40 ° C and wait until the unit heats up. Once the incubator has warmed up to the operating temperature, you can place the sample well in the appropriate hole.
 
 
Carry out a self-test of the test set:
 
 
Carry out a self-test of the test set before working with the samples. The negative and positive test samples included in the kit should be used for this purpose. Further work on the kit, with confirmed antibiotic detection characteristics and limits, is permitted if a completely negative control sample is obtained and a completely positive control sample is obtained.
 
Preparation of a negative control sample:
 
 
Add 2 ml of distilled or deionized water to a bottle containing a negative control sample and mix well. Store the prepared negative standard at 2-8 °C for a maximum of 24 hours. Frozen negative sample should be kept in refrigerator for not more than 30 days at temperature equal or lower than - 16 oÑ. Before use, the negative control should be brought to room temperature and thoroughly mixed. Follow the milk and whey analysis procedure (read more).
 
 
Prepare a positive control sample:
 
 
Add 200 µl of recovered negative control sample to the reagent well containing the positive control sample. Use a pipette to mix them thoroughly. After mixing, the sample mixture and reagent from the well should have a homogeneous structure. Place the well with the pink reagent containing antibodies in an incubator or well plate preheated to 40 °C. Select 200 µl of the resulting positive control sample with a pipette and transfer it to the well with the pink reagent, using a pipette, stir it 10 times until it is evenly pink colored. Incubate the mixture for 3 minutes. Then place the test strip from the kit in the sample well and incubate for another 5 minutes.
 
After incubation, remove the test strip from the sample well and interpret the result for 3 minutes.
 
 
If the test set is stored correctly, internal quality control using positive and negative standards should only be performed once before starting the tests at the first opening of the test set box.
 
 
Milk and whey analysis procedure:
 
 
Read the user manual before analyzing. Determine how many tests you need, remove the required number of tests from the refrigerator. The reagents in the kit should be at room temperature. Reagents and test strips from open tubes should preferably be used within 24 hours of being removed from the refrigerator to avoid distorted results. All unused reagents of the kit should be removed from the fridge. Avoid direct sunlight and excessive moisture on the reagents. The reagent in the well is specially dried. Do not think that it is spoiled.
 
 
When using an incubator, place the sample well in a preheated incubator to 40 °C, use a pipette to take a sample of 200 µl of milk or whey and transfer it to the reagent well from the set. Thoroughly mix the sample with the reagent from the kit ten times and drain the liquid using a pipette in the well until the pink colouring is even and incubate the mixture for 3 minutes.
Then place the test strip from the kit in the reagent wells and sample and incubate in the incubator for another 5 minutes at 40 °C.
 
 
In the absence of an incubator, the test can be done indoors at 16-25 °C. Place the sample well in the well pad, use a pipette to take a sample of 200 µl of milk or whey and transfer it to the reagent well from the kit. Thoroughly mix the sample with the reagent from the ten-pack and drain the liquid using the pipette in the well until the pink coloration is even. Incubate the mixture for 3 minutes.
Then place a test strip from the kit in the reagent wells and sample and incubate for 5 more minutes.
 
 
After incubation, remove the test strip from the reagent well and sample and visually interpret the result for 3 minutes or read it with the BMZ 6000 reader (if available). To save the result, remove the filter from the bottom end.
test strips.
 
Visual interpretation of the result:
 
 
Note:
 
 
Test strip is a qualitative method of detection of residual antibiotics in milk, milk serum.
Do not use damaged kit components or expired kits.
 
 
After removing the kit from the fridge, it is advisable to use the opened components within 24 hours.
 
 
Hold the test strip only by the upper end. Do not touch the work surface filter of the test strip! Do not touch the diaphragm in the middle of the test strip!
Close the tube tightly after removing the required number of test strips and wells.
Place the test strips in the wells of the working area with the filter.
 
 
Each time a new sample is taken, take a new disposable pipette tip to avoid accidental contamination.
 
 
The test strip is only used once.
 
 
Milk and whey samples and the mixture of sample and dye in the well should be homogeneous. Examining samples of milk and whey with foreign particles, clots and separation phases can lead to distorted results.
If necessary, it is possible to store test strips with results not exceeding 12 months.
 
 
One test strip and one reagent well are used per sample, the remaining test strips and wells are stored in a closed tube at 2-8 °C.

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